Title

Facilitated Tau Degradation by USP14 Aptamers via Enhanced Proteasome Activity.

Journal

Scientific reports

Volume

5

Issue

None

Pages

10757

Date

2015-06-04

Authors

Choi WH | Hong C | Jiang Y | Kim DE | Lee JH | Lee MJ | Shin SK

We identified three specific RNA aptamers of USP14 (USP14-1, USP14-2, and USP14-3) that inhibited its deubiquitinating activity. The nucleotide sequences of these non-cytotoxic USP14 aptamers contained conserved GGAGG motifs, with G-rich regions upstream, and similar secondary structures. They efficiently elevated proteasomal activity, as determined by the increased degradation of small fluorogenic peptide substrates and physiological polyubiquitinated Sic1 proteins. Additionally, proteasomal degradation of tau proteins was facilitated in the presence of the UPS14 aptamers in vitro.

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