After nicotine treat- ment for 24 h at 30µM nicotine, 125 I-labeled epibatidine bind- ing to a6b2 receptors increased significantly at 37 and 30°C.
This range is broader but similar to what was observed for a4b2 where the -fold increase after nicotine treat- ment varied 3–6-fold (29).
At 37°C, the timecourse of the a6b2 and a3b2 up-regulation is essentially complete after 2 h, and no signifi- cant changes were observed with longer nicotine incubations.
We first assayed expression of a6b2 or a3b2 receptors by binding the agonist, 125 I-labeled epibatidine (Fig. 1, A and B), to stably transfected a6FLAGb2HA cells and transiently trans- fected a3FLAGb2HA cells.
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.