PubMed: 18174175

Title
Up-regulation of nicotinic receptors by nicotine varies with receptor subtype.
Journal
The Journal of biological chemistry
Volume
283
Issue
None
Pages
6022-32
Date
2008-03-07
Authors
Vallejo Y | Hoda JC | Govind AP | Mastro R | Walsh H | Green WN | Bertrand D

Evidence b56f956165

After nicotine treat- ment for 24 h at 30µM nicotine, 125 I-labeled epibatidine bind- ing to a6b2 receptors increased significantly at 37 and 30°C.

Evidence 8682bfdef8

This range is broader but similar to what was observed for a4b2 where the -fold increase after nicotine treat- ment varied 3–6-fold (29).

Evidence 317641301f

At 37°C, the timecourse of the a6b2 and a3b2 up-regulation is essentially complete after 2 h, and no signifi- cant changes were observed with longer nicotine incubations.

Evidence 1b0bb948f7

We first assayed expression of a6b2 or a3b2 receptors by binding the agonist, 125 I-labeled epibatidine (Fig. 1, A and B), to stably transfected a6FLAGb2HA cells and transiently trans- fected a3FLAGb2HA cells.

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