Title

Glycogen synthase kinase-3beta is complexed with tau protein in brain microtubules.

Journal

The Journal of biological chemistry

Volume

277

Issue

None

Pages

11933-40

Date

2002-04-05

Authors

Cafferty PW | Sobue K | Qureshi HY | Neufield KD | Sun W | Agarwal-Mawal A | Paudel HK

GSK3Beta may directly bind to tau within a GSK3Beta-tau complex.To identify the GSK3Beta-binding region within tau, we constructed three tau deletion mutants: R-tau-(1–244) containing the N-terminal projection domain, R-tau-(245–369) containing the microtubule-binding region, and R-tau-(245–441) containing both the microtubule-binding region and the C-terminal tail. We evaluated the binding of these mutants and wild-type R-tau with GST-GSK3Beta by the GST pull-down assay. GSK3Beta bound to wild type (Fig. 5B) and R-tau-(1–244) (Fig. 5C) but not R-tau-(245–369) (Fig. 5D) and R-tau-(245–441) (Fig.5E). These data demonstrate that GSK3Beta binds to the Nterminal projection domain of tau.

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