Aorta
By-products of heme and iron metabolism, such as heme oxygenase-dependent generation of carbon monoxide (CO), have antioxidant and anti-inflammatory protective functions against iron-induced vascular damage (33); therefore we examined the impact of CO donors on collagen exposure and thrombus formation in the ex vivo vascular injury model. PubMed:19276082
Pretreating anticoagulated whole blood with tricarbonyldichlororuthenium(II) dimer, a CO donor, significantly reduced collagen exposure (Fig. 5A) and thrombus formation (Fig. 5B) induced by FeCl3. PubMed:19276082
Analysis of the time course of hemolysis in whole blood revealed a rapid linear increase in Hb levels, peaking 10 min after FeCl3 addition (Fig. 2B), a time course consistent with the rapid hemolysis and vascular injury observed in the ex vivo aortic thrombosis model. PubMed:19276082
To investigate the direct effects of FeCl3 on aortic endothelium, FeCl3 was perfused through isolated aorta independently of flowing blood. Strikingly no endothelial denudation or collagen exposure was evident following prolonged exposure to FeCl3 (6%) (Fig. 1C). PubMed:19276082
Analysis of Hb release from ex vivo vessel chamber experiments revealed a significant (p 0.05; n 4) increase in Hb levels following FeCl3 treatment of isolated aorta in the presence of flowing blood (157 45 g/ml), whereas FeCl3 pretreatment of vessels prior to blood perfusion caused no hemolysis (Fig. 2A). PubMed:19276082
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.