complex(a(MESH:Proteins), a(MESH:Ubiquitin))

Components

• a(MESH:Proteins)
• a(MESH:Ubiquitin)

Another key early finding was that the cleavage of the ubiquitin chain from the substrate was ATP-dependent and was coupled to the translocation of the protein substrate into the 20S core PubMed:24457024

These enzymes, referred to as deubiquitinases (DUBs), cleave ubiquitin from conjugated proteins or edit ubiquitin chains by trimming their lengths [62] PubMed:24457024

This observation suggests that chain binding and cleavage from the protein substrate are tightly coordinated by the proteasome, and may help to prevent situations such as premature chain cleavage by Rpn11, which could result in the release of the substrate before it becomes actively engaged to the proteasome. PubMed:24457024

Second, Rpn11/Poh1 (also known as Psmd14) is a subunit in the 19S regulatory particle that cleaves the entire, intact ubiquitin chain from the protein substrate [71,72]. PubMed:24457024

This observation suggests that chain binding and cleavage from the protein substrate are tightly coordinated by the proteasome, and may help to prevent situations such as premature chain cleavage by Rpn11, which could result in the release of the substrate before it becomes actively engaged to the proteasome. PubMed:24457024

Following degradation of the substrate, short peptides are released along with free and reusable ubiquitin. PubMed:14556719

Following degradation of the substrate, short peptides are released along with free and reusable ubiquitin. PubMed:14556719