path(MESH:"Memory Disorders")
We did not detect any glia or microglia activation in WT-APP (Fig. 3C and F) compared with WT-GFP (Fig. 3B and E), meaning that the neuroinflammation does not play a role in the memory deficit we observed PubMed:27522251
The hAPP-SLA transduction in DG did not induce a memory deficit in beta2 KO, meaning that the Abeta/beta2-nAChR interaction is required to drive the memory deficit in this model PubMed:27522251
The hAPP-SLA transduction in DG did not induce a memory deficit in beta2 KO, meaning that the Abeta/beta2-nAChR interaction is required to drive the memory deficit in this model PubMed:27522251
In the NPR task (7 months p.i.), the GFP-beta2 spent significantly more time exploring the novel compartment (p = 0.003; Fig. 4A), as well as APP-beta2 (p = 0.017; Fig. 4B). PubMed:27522251
At 7 months p.i., both GFP-alpha7 (p = 0.9; Fig. 5A) and APP-alpha7 groups (p = 0.3; Fig. 5B) displayed a memory deficit in the NPR task. PubMed:27522251
The analysis of the total exploration time during the NOR task showed that GFP-alpha7 spent similar time exploring the familiar and the novel objects (p = 0.76; Fig. 5D), whereas APP-alpha7 spent significantly more time exploring the novel object compared with the familiar one (p = 0.01; Fig. 5E) PubMed:27522251
However, the analysis of the recognition percentage for the same set of data showed that GFP-WT spent significantly more time exploring the novel object compared with APP-WT (p = 0.0032; Fig. 2E), suggesting the presence of a memory deficit in the APP-WT group. PubMed:27522251
Hence, the transduction of hAPP-SLA in DG induces recognition memory deficits as supported by NPR and NOR tasks PubMed:27522251
Similarly, in the NOR task (15 months p.i.), both the GFP-beta2 and the APP-beta2 displayed higher exploration of the novel object (p < 0.0001 and p = 0.0001, respectively; Fig. 4C and D), with no differences between the 2 groups in the recognition index (p = 0.7; Fig. 4E), meaning that beta2 mice injected with hAPP-SLA did not exhibit the recognition memory deficit observed in APP-WT PubMed:27522251
Noninjected mice spent the same amount of time exploring novel and familiar compartments during the test session (p = 0.6; Fig. 5C), confirming the presence of a constitutive recognition memory deficit in age-matched alpha7 KO mice PubMed:27522251
Because the alpha7 KO had a memory deficit independent of hAPP-SLA expression, we could not draw any conclusion on the role of alpha7 in the memory deficit observed PubMed:27522251
In the NPR task (7 months p.i.), the GFP-beta2 spent significantly more time exploring the novel compartment (p = 0.003; Fig. 4A), as well as APP-beta2 (p = 0.017; Fig. 4B). PubMed:27522251
Similarly, in the NOR task (15 months p.i.), both the GFP-beta2 and the APP-beta2 displayed higher exploration of the novel object (p < 0.0001 and p = 0.0001, respectively; Fig. 4C and D), with no differences between the 2 groups in the recognition index (p = 0.7; Fig. 4E), meaning that beta2 mice injected with hAPP-SLA did not exhibit the recognition memory deficit observed in APP-WT PubMed:27522251
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.