p(MGI:P2rx4)
These results strongly suggest that heme activates NLRP3 through P2X receptors, especially P2X4R and P2X7R. PubMed:24464629
Therefore, knockdown of P2X4R and double knockdown of P2X7R plus P2X4R were performed by shRNA in macrophages and the result showed that P2X4R knockdown also partially inhibit the heme induced IL-1b secretion and double knockdown of P2X7R plus P2X4R almost completely depressed the IL-1b secretion (Fig. 3d). PubMed:24464629
These results strongly suggest that heme activates NLRP3 through P2X receptors, especially P2X4R and P2X7R. PubMed:24464629
Given that P2X4 and P2X7 are the major purinergic P2X receptor subtypes, a study of spinal cord injury in P2X4 knock-out mice showed a significant reduction in inflammasome activation and proinflammatory cytokine production as compared to wild type (de Rivero Vaccari et al., 2012), supporting the involvement of purinergic receptor P2X4 in the activation of the NLRP1 inflammasome PubMed:24561250
However, upon further study of purinergic receptor P2X4 knockout mice with spinal cord injury, the production of IL-1β but not of IL-18 reduces in the neurons as compared with wild-type mice (de Rivero Vaccari et al., 2012) PubMed:24561250
P2X4 knock-out mice has been shown to decrease the level of IL-1β and to have impair inflammasome signaling (de Rivero Vaccari et al., 2012) PubMed:24561250
P2X4 knock-out mice has been shown to decrease the level of IL-1β and to have impair inflammasome signaling (de Rivero Vaccari et al., 2012) PubMed:24561250
Therefore, knockdown of P2X4R and double knockdown of P2X7R plus P2X4R were performed by shRNA in macrophages and the result showed that P2X4R knockdown also partially inhibit the heme induced IL-1b secretion and double knockdown of P2X7R plus P2X4R almost completely depressed the IL-1b secretion (Fig. 3d). PubMed:24464629
These results strongly suggest that heme activates NLRP3 through P2X receptors, especially P2X4R and P2X7R. PubMed:24464629
These results strongly suggest that heme activates NLRP3 through P2X receptors, especially P2X4R and P2X7R. PubMed:24464629
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.