embryonic cell line
Final cumulative mortality (at 10 h recovery) in non-heat shocked embryos was low in both the 0 nM and 10 nM antalarmin groups (4.7% and 8.3%, respectively), and heat shock increased mortality in both groups PubMed:29807032
CRF overexpression by mRNA microinjection reduced heat shock-induced caspase-3 activity by approximately 2-fold relative to control-injected embryos PubMed:29807032
The greatest heat shock-induced mortality occurred during the first 1 h of recovery and plateaued after 5 h recovery (main effect of time P<0.001; n=3; Fig. 3B), but the presence of antalarmin did not influence this response. PubMed:29807032
However, embryos that recovered from heat shock in the presence of the CRF-R1 antagonist, antalarmin, experienced a greater increase in caspase-3 activity than embryos not given the antagonist PubMed:29807032
In embryos heat shocked at 6 hpf, crf abundance increased relative to controls after 4 h recovery, but there were no differences at 7 h or 10 h recovery (Fig. 1A). PubMed:29807032
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.