complex(a(MESH:Ubiquitin), p(HGNC:UBA1))
Initially, the ubiquitin-activating enzyme E1 activates ubiquitin in an ATP-requiring reaction to generate a high-energy thiol ester intermediate, E1-S~ubiquitin, where ubiquitin is bound to an internal E1 Cys residue PubMed:14556719
One of several E2 enzymes (ubiquitin-carrier proteins or Ubiquitin-Conjugating enzymes [UBCs]) transfers the activated ubiquitin moiety from E1, via an additional high-energy thiol ester intermediate, E2-S~ubiquitin, to the substrate that is specifically bound to an E3, a member of the ubiquitinprotein ligase family of proteins PubMed:14556719
The thioesterified ubiquitin passes from the E1 active site to the next member of the cascade, the E2 or ubiquitin-conjugating enzyme PubMed:24457024
E1s are multidomain enzymes that must activate ubiquitin and efficiently transfer it to the E2 active site PubMed:24457024
BEL Commons is developed and maintained in an academic capacity by Charles Tapley Hoyt and Daniel Domingo-Fernández at the Fraunhofer SCAI Department of Bioinformatics with support from the IMI project, AETIONOMY. It is built on top of PyBEL, an open source project. Please feel free to contact us here to give us feedback or report any issues. Also, see our Publishing Notes and Data Protection information.
If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.