p(HGNC:SERPINA1)
Under the same experimental conditions, A1AT blocked the ability of hemin to reduce GR activity (Fig. 10). PubMed:28716864
However, in the presence of A1AT, hemin did not change PKC activity significantly. PubMed:28716864
Under the same experimental conditions, addition of 1 mg/ ml A1AT significantly prevented hemin-induced neutrophil spreading and adhesion (Fig. 3A). PubMed:28716864
Under the same experimental conditions, addition of 1 mg/ ml A1AT significantly prevented hemin-induced neutrophil spreading and adhesion (Fig. 3A). PubMed:28716864
Based on the previous findings that hemin induces neutrophil adhesion to endothelial cells [8] and that A1AT protects endothelial cells from neutrophil adhesion induced by fMLP [27], we investigated whether A1AT, as a scavenger of hemin, can prevent hemin-induced neutrophil adhesion to HUVECs. As shown in Fig. 4, neutrophils treated with hemin or fMLP (used as a positive control) exhibited a 3-fold higher adhesion to HUVECs compared with controls. However, the adherence of neutrophils treated with hemin/A1AT did not differ from controls (Fig. 4). PubMed:28716864
In the presence of A1AT, this latter effect of hemin was significantly inhibited and did not differ from controls (Fig. 5B). PubMed:28716864
In the presence of A1AT, hemin-induced release of IL-8 protein was inhibited significantly (Fig. 7B). PubMed:28716864
The ability of hemin to trigger ROS production in neutrophils was abrogated significantly in the presence of A1AT (Fig. 8). PubMed:28716864
In the presence of A1AT, hemin effect on HMOX1 expression was diminished significantly (Fig. 9A). PubMed:28716864
Under the same experimental conditions, A1AT blocked the ability of hemin to reduce GR activity (Fig. 10). PubMed:28716864
However, in the presence of A1AT, hemin did not change PKC activity significantly. PubMed:28716864
The main function of A1AT is to inhibit neutrophil elastase and proteinase 3. PubMed:28716864
The main function of A1AT is to inhibit neutrophil elastase and proteinase 3. PubMed:28716864
However A1AT has broader functions [26, 27], abrogating inflammation via both enzyme-inhibitory and noninhibitory mechanisms [47]. PubMed:28716864
Along with this, A1AT inhibited hemin to induce PKC phosphorylation, which is an essential step for the production of ROS [9]. PubMed:28716864
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.