composite(a(HBP:"Tau aggregates"), p(HGNC:MAPT, var("p.S422E")))

Components

• a(HBP:"Tau aggregates")
• p(HGNC:MAPT, var("p.S422E"))

Compared to monomers, aggregation significantly increased PAD exposure for both hT40 and S422E samples (Fig. 3B; F(1,12) = 685.8, p b 0.0001), as indicated by increased TNT1 reactivity. Aggregation also significantly increased oligomer formation (TOC1 reactivity) compared to monomers in both hT40 and S422E samples (Fig. 3C; F(1,12) = 109.3, p b 0.0001). PubMed:27373205

In contrast, aggregation of either hT40 or S422E significantly increased the amount of SDS-stable dimers at 180 kDa (Fig. 4C; F(1,12) = 110.0; p b 0.0001), as compared to the levels of these species in the respective monomer samples. Furthermore, S422E aggregation significantly increased the amount of SDS-stable dimers compared to hT40 aggregation (p = 0.03). PubMed:27373205

In contrast to hT40 monomer, perfusion of S422E monomer selectively inhibited anterograde transport (Fig. 5C; Fig. 6A; p = 0.028), but not retrograde FAT. Surprisingly, aggregated S422E significantly inhibited both anterograde and retrograde FAT rates (Fig. 5D; Fig. 6A, B) compared to S422E monomer (anterograde, p = 0.012; retrograde, p = 0.002) and hT40 aggregates (retrograde only, p = 0.019). PubMed:27373205

In contrast to hT40 monomer, perfusion of S422E monomer selectively inhibited anterograde transport (Fig. 5C; Fig. 6A; p = 0.028), but not retrograde FAT. Surprisingly, aggregated S422E significantly inhibited both anterograde and retrograde FAT rates (Fig. 5D; Fig. 6A, B) compared to S422E monomer (anterograde, p = 0.012; retrograde, p = 0.002) and hT40 aggregates (retrograde only, p = 0.019). PubMed:27373205