Muscle, Smooth, Vascular
In VSMC pretreated with the ERK-2 inhibitor PD98059 (10 mM), migration induced by heme was partially inhibited (Fig. 3A) and heme proliferative effect on VSMC was impaired (Fig. 3B). PubMed:22954673
In VSMC pretreated with the ERK-2 inhibitor PD98059 (10 mM), migration induced by heme was partially inhibited (Fig. 3A) and heme proliferative effect on VSMC was impaired (Fig. 3B). PubMed:22954673
Fig. 4 shows that the inhibition of HO activity by both, SnPP (Fig. 4A) or ZnPP (Fig. 4B), potentiates the effect of heme on VSMC proliferation. PubMed:22954673
Additionally, it was observed that inhibition of HO-1 by ZnPP induced a further increase in hemeinduced ROS production (Fig. 4C), suggesting that activation of HO system attenuates the NADPHox-dependent activation of VSMC induced by heme. PubMed:22954673
Heme effects were shown to be redoxsensitive, since the antioxidant Trolox (100 mM) abrogated heme-induced VSMC migration and proliferation (Fig. 1C,D). PubMed:22954673
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.