g(DBSNP:rs63751011)
Four weeks after terminal differentiation, 80% of cells were positive for the neuron-specific marker MAP2, and there was no significant difference between control and patient neurons (Figure S2A) PubMed:27594586
The percentages of VGLUT1-positive excitatory and GABA-positive inhibitory neurons were similar in neuronal cultures differentiated from control and patient iPSCs (Figures S2B and S2C) PubMed:27594586
The percentages of VGLUT1-positive excitatory and GABA-positive inhibitory neurons were similar in neuronal cultures differentiated from control and patient iPSCs (Figures S2B and S2C) PubMed:27594586
Moreover, the total expression levels of tau and PSD-95 were the same in 1-month-old neurons derived from control and patient iPSCs (Figure 1B) PubMed:27594586
Moreover, the total expression levels of tau and PSD-95 were the same in 1-month-old neurons derived from control and patient iPSCs (Figure 1B) PubMed:27594586
The resting membrane potential did not differ among neurons differentiated from all iPSC lines (Figure S2D) PubMed:27594586
Thus, both molecular and electrophysiological analyses suggest MAPT mutations do not affect early neuronal differentiation PubMed:27594586
Tau-A152T neurons had >10-fold higher level and activity of secreted MMP-9 than control neurons (Figures 2A and 2B), as did MAPT IVS10+16 neurons to a lesser extent (Figures 2C and 2D) PubMed:27594586
Tau-A152T neurons had >10-fold higher level and activity of secreted MMP-9 than control neurons (Figures 2A and 2B), as did MAPT IVS10+16 neurons to a lesser extent (Figures 2C and 2D) PubMed:27594586
This increase is likely in part due to transcriptional regulation, since the mRNA levels of MMP-9 and MMP-2 in tau-A152T and MAPT IVS10+16 neurons were higher than in control neurons (Figure S2J) PubMed:27594586
MMP-9/MMP-2 mRNA levels in tau-A152T and MAPT IVS10+16 neurons were significantly higher than that in control neurons (Figure S2J), and rapamycin increased these levels (p < 0.01) (Figure 3C) PubMed:27594586
This increase is likely in part due to transcriptional regulation, since the mRNA levels of MMP-9 and MMP-2 in tau-A152T and MAPT IVS10+16 neurons were higher than in control neurons (Figure S2J) PubMed:27594586
MMP-9/MMP-2 mRNA levels in tau-A152T and MAPT IVS10+16 neurons were significantly higher than that in control neurons (Figure S2J), and rapamycin increased these levels (p < 0.01) (Figure 3C) PubMed:27594586
We found that, indeed, TUJ1+ neurons showed reduced survival as measured by TUNEL analysis (Figure 3A) PubMed:27594586
As expected, the 4R/3R tau ratio is greatly increased in MAPT IVS10+16 neurons (Figure S3F) but not in GRN mutant neurons (Figure S3G) PubMed:27594586
As expected, the 4R/3R tau ratio is greatly increased in MAPT IVS10+16 neurons (Figure S3F) but not in GRN mutant neurons (Figure S3G) PubMed:27594586
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.