bp(GO:"actin cytoskeleton reorganization")
Tau thereby stabilizes microtubules, promotes microtubule assembly and, in particular, regulates the dynamic instability of microtubules that allows reorganization of the cytoskeleton. PubMed:26631930
We quantified these changes by automatic image analysis and found that heme-induced cytoskeleton rearrangement led to a significant increase in cell area and perimeter, as well as a decrease in circularity (form factor) (Fig. 4d,e and Supplementary Fig. 3b–d), indicating that the heme-induced defective phagocytic response was likely tied to cytoskeleton rearrangements. PubMed:27798618
Quinine pretreatment protected RAW264.7 macrophages (Fig. 8b and Supplementary Fig. 8c,d) and human macrophages (Fig. 8c) from heme-induced inhibition of phagocytosis and actin cytoskeleton changes (Supplementary Fig. 8e,f) compared with DMSO-treated cells. PubMed:27798618
We quantified these changes by automatic image analysis and found that heme-induced cytoskeleton rearrangement led to a significant increase in cell area and perimeter, as well as a decrease in circularity (form factor) (Fig. 4d,e and Supplementary Fig. 3b–d), indicating that the heme-induced defective phagocytic response was likely tied to cytoskeleton rearrangements. PubMed:27798618
Together, these data indicate that heme induces extensive actin cytoskeleton alterations, which results in defective phagocytosis and inflammatory cell migration. PubMed:27798618
Our finding that DOCK8 is necessary for the cytoskeleton changes and disruption of bacterial phagocytosis by heme is consistent with recent studies showing that DOCK8 regulates dendritic cell migration via Cdc42 (refs. 37,44). PubMed:27798618
We quantified these changes by automatic image analysis and found that heme-induced cytoskeleton rearrangement led to a significant increase in cell area and perimeter, as well as a decrease in circularity (form factor) (Fig. 4d,e and Supplementary Fig. 3b–d), indicating that the heme-induced defective phagocytic response was likely tied to cytoskeleton rearrangements. PubMed:27798618
We quantified these changes by automatic image analysis and found that heme-induced cytoskeleton rearrangement led to a significant increase in cell area and perimeter, as well as a decrease in circularity (form factor) (Fig. 4d,e and Supplementary Fig. 3b–d), indicating that the heme-induced defective phagocytic response was likely tied to cytoskeleton rearrangements. PubMed:27798618
Together, these data indicate that heme induces extensive actin cytoskeleton alterations, which results in defective phagocytosis and inflammatory cell migration. PubMed:27798618
Quinine pretreatment protected RAW264.7 macrophages (Fig. 8b and Supplementary Fig. 8c,d) and human macrophages (Fig. 8c) from heme-induced inhibition of phagocytosis and actin cytoskeleton changes (Supplementary Fig. 8e,f) compared with DMSO-treated cells. PubMed:27798618
Our finding that DOCK8 is necessary for the cytoskeleton changes and disruption of bacterial phagocytosis by heme is consistent with recent studies showing that DOCK8 regulates dendritic cell migration via Cdc42 (refs. 37,44). PubMed:27798618
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If you find BEL Commons useful in your work, please consider citing: Hoyt, C. T., Domingo-Fernández, D., & Hofmann-Apitius, M. (2018). BEL Commons: an environment for exploration and analysis of networks encoded in Biological Expression Language. Database, 2018(3), 1–11.