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PubMed: 27125525

Title
Hematoma Changes During Clot Resolution After Experimental Intracerebral Hemorrhage.
Journal
Stroke
Volume
47
Issue
None
Pages
1626-31
Date
2016-06-01
Authors
Cao S | Chen G | Hua Y | Keep RF | Xi G | Zheng M

Evidence 5be010ab33
JSON

The diameter of RBCs within the hematoma decreased with time after ICH.

bp(MESH:"Erythrocyte Indices") negativeCorrelation path(MESH:Hematoma) View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Discussion

path(MESH:Hematoma) negativeCorrelation bp(MESH:"Erythrocyte Indices") View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Discussion

Evidence 3d8110807d
JSON

We found that hemoglobin contents in hematoma were significantly higher in the DFX treated group compare to the vehicle-treated group (15.9 ± 1.8 vs. 11.8 ± 0.8 mg/g, p<0.05, Fig. 5A).

a(CHEBI:"desferrioxamine B") positiveCorrelation p(HGNC:HBB) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

p(HGNC:HBB) negativeCorrelation path(MESH:Hematoma) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

p(HGNC:HBB) positiveCorrelation a(CHEBI:"desferrioxamine B") View Subject | View Object

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Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

path(MESH:Hematoma) negativeCorrelation p(HGNC:HBB) View Subject | View Object

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MeSH
Cerebral Hemorrhage
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Results

Evidence 21ab602b7e
JSON

DFX also reduced the MAC content in the clot at day-3 (33.0 ± 6.6 vs. 56.2 ± 9.2 ng/g in the vehicle-treated group, p<0.05, Fig. 5B).

a(CHEBI:"desferrioxamine B") negativeCorrelation a(MESH:"Complement Membrane Attack Complex") View Subject | View Object

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MeSH
Cerebral Hemorrhage
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Results

a(MESH:"Complement Membrane Attack Complex") negativeCorrelation a(CHEBI:"desferrioxamine B") View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence 5f740aaa72
JSON

The present study found that DFX treatment reduces MAC formation in the clot.

a(CHEBI:"desferrioxamine B") negativeCorrelation a(MESH:"Complement Membrane Attack Complex") View Subject | View Object

Appears in Networks:
Annotations
Cell Ontology (CL)
macrophage
MeSH
Stroke
Text Location
Discussion

a(MESH:"Complement Membrane Attack Complex") negativeCorrelation a(CHEBI:"desferrioxamine B") View Subject | View Object

Appears in Networks:
Annotations
Cell Ontology (CL)
macrophage
MeSH
Stroke
Text Location
Discussion

Evidence 91e6da39e9
JSON

These results suggested that the treatment of DFX reduced the process of hemolysis after ICH, which might be due to alleviating MAC formation.

a(CHEBI:"desferrioxamine B") decreases path(MESH:Hemolysis) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence 6f12225c03
JSON

The loss of CD47 in the clot was reduced significantly by DFX treatment at day 3 (CD47/GAPDH: 1.32 ± 0.16 in ICH+DFX group vs. 0.74 ± 0.07 in ICH + vehicle group, p<0.05, Fig. 5C) and day 7 (p<0.01, Suppl Figure I).

a(CHEBI:"desferrioxamine B") positiveCorrelation p(HGNC:CD47) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

p(HGNC:CD47) positiveCorrelation a(CHEBI:"desferrioxamine B") View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence e446c7ec44
JSON

DFX also reduced CD47 loss and microglia/macrophage infiltration after ICH suggesting it also affects erythrophagocytosis.

a(CHEBI:"desferrioxamine B") positiveCorrelation p(HGNC:CD47) View Subject | View Object

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Cell Ontology (CL)
macrophage
MeSH
Stroke
Text Location
Discussion

p(HGNC:CD47) positiveCorrelation a(CHEBI:"desferrioxamine B") View Subject | View Object

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Cell Ontology (CL)
macrophage
MeSH
Stroke
Text Location
Discussion

Evidence 417a624382
JSON

DFX treatment also caused a significant reduction in infiltrating CD163-positive and HO-1 positive cell in the hematoma at day 3 (Figs. 6A and B) and day 7 (Suppl Figures II & III).

a(CHEBI:"desferrioxamine B") negativeCorrelation p(HGNC:CD163) View Subject | View Object

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MeSH
Cerebral Hemorrhage
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Results

a(CHEBI:"desferrioxamine B") negativeCorrelation p(HGNC:HMOX1) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

p(HGNC:CD163) negativeCorrelation a(CHEBI:"desferrioxamine B") View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

p(HGNC:HMOX1) negativeCorrelation a(CHEBI:"desferrioxamine B") View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence 1c2a2e5ba5
JSON

Western blot analysis showed that the protein expression of HO-1 was decreased in the hematoma in the DFX treated group at day 3( HO-1/GAPDH: 0.32 ± 0.04 vs. 0.98 ± 0.07 in the vehicle-treated group, p<0.05, Fig. 6B) and day 7 (p<0.05, Suppl Figure III).

a(CHEBI:"desferrioxamine B") negativeCorrelation p(HGNC:HMOX1) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

p(HGNC:HMOX1) negativeCorrelation a(CHEBI:"desferrioxamine B") View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence 0972c21e2e
JSON

In combination, these finding may underlie our previous results showing that DFX slows hematoma resolution after ICH in aged rats 12.

a(CHEBI:"desferrioxamine B") negativeCorrelation path(MESH:Hematoma) View Subject | View Object

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Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
Text Location
Discussion

path(MESH:Hematoma) negativeCorrelation a(CHEBI:"desferrioxamine B") View Subject | View Object

Appears in Networks:
Annotations
Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
Text Location
Discussion

Evidence eeefa0b10f
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CD163 mediated hemoglobin/hematoma clearance is involved in the induction of HO-1, a rate-limiting enzyme for heme degradation 26.

deg(a(CHEBI:heme)) positiveCorrelation p(HGNC:HMOX1) View Subject | View Object

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Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
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Discussion

p(HGNC:CD163) increases p(HGNC:HMOX1) View Subject | View Object

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Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
Text Location
Discussion

p(HGNC:HMOX1) positiveCorrelation deg(a(CHEBI:heme)) View Subject | View Object

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Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
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Discussion

Evidence c077f16923
JSON

Erythrophagocytosis was observed in the hematoma edge at day-3 (Fig. 3A), and hemosiderin deposition was identifiable in the hematoma edge at day-7 (Fig. 3A).

a(HM:erythrophagocytosis) positiveCorrelation path(MESH:Hematoma) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

a(MESH:Hemosiderin) positiveCorrelation path(MESH:Hematoma) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

path(MESH:Hematoma) positiveCorrelation a(HM:erythrophagocytosis) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

path(MESH:Hematoma) positiveCorrelation a(MESH:Hemosiderin) View Subject | View Object

Appears in Networks:
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MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence f05f4088f0
JSON

CD47 exerts its inhibitory effect on phagocytosis through binding to inhibitory immunoreceptor SIRPα expressed by microglia/macrophages 21.Thus, the CD47-SIRPα constitutes a negative feedback for erythrophagocytosis22.

a(HM:erythrophagocytosis) negativeCorrelation complex(p(HGNC:CD47), p(HGNC:SIRPA)) View Subject | View Object

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Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
Text Location
Discussion

complex(p(HGNC:CD47), p(HGNC:SIRPA)) negativeCorrelation a(HM:erythrophagocytosis) View Subject | View Object

Appears in Networks:
Annotations
Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
Text Location
Discussion

Evidence a9dfbb1f13
JSON

Evidence suggested that CD163 acts as a hemoglobin scavenger receptor on microglia/macrophage with a role in erythrophagocytosis.

a(HM:erythrophagocytosis) positiveCorrelation p(HGNC:CD163) View Subject | View Object

Appears in Networks:
Annotations
Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
Text Location
Discussion

p(HGNC:CD163) positiveCorrelation a(HM:erythrophagocytosis) View Subject | View Object

Appears in Networks:
Annotations
Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
Text Location
Discussion

Evidence 6f6198b580
JSON

A recent study, however, indicated that HO-1 is associated with blood clearance by enhancing erythrophagocytosis after stroke.

a(HM:erythrophagocytosis) positiveCorrelation p(HGNC:HMOX1) View Subject | View Object

Appears in Networks:
Annotations
Cell Ontology (CL)
macrophage
MeSH
Stroke
Text Location
Discussion

p(HGNC:HMOX1) positiveCorrelation a(HM:erythrophagocytosis) View Subject | View Object

Appears in Networks:
Annotations
Cell Ontology (CL)
macrophage
MeSH
Stroke
Text Location
Discussion

Evidence eb32854d4f
JSON

Erythrocyte lysis after ICH can be mediated by the complement activation and formation of the membrane attack complex (MAC), which contains complement C5b, C6, C7, C8 and C9 proteins (C5b– 9).

a(MESH:"Complement Membrane Attack Complex") positiveCorrelation path(MESH:Hemolysis) View Subject | View Object

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Text Location
Introduction

bp(GO:"complement activation") positiveCorrelation path(MESH:Hemolysis) View Subject | View Object

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Introduction

path(MESH:Hemolysis) positiveCorrelation bp(GO:"complement activation") View Subject | View Object

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Text Location
Introduction

path(MESH:Hemolysis) positiveCorrelation a(MESH:"Complement Membrane Attack Complex") View Subject | View Object

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Introduction

Evidence c23de78734
JSON

Activation of the complement system and the formation of MAC resulted in an increased membrane permeability and erythrocyte lysis.

bp(GO:"complement activation") positiveCorrelation path(MESH:Hemolysis) View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

a(MESH:"Complement Membrane Attack Complex") positiveCorrelation path(MESH:Hemolysis) View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

a(MESH:"Complement Membrane Attack Complex") positiveCorrelation bp(MESH:"Cell Membrane Permeability") View Subject | View Object

Appears in Networks:
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MeSH
Cerebral Hemorrhage
Text Location
Results

bp(GO:"complement activation") positiveCorrelation bp(MESH:"Cell Membrane Permeability") View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

bp(MESH:"Cell Membrane Permeability") positiveCorrelation bp(GO:"complement activation") View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

bp(MESH:"Cell Membrane Permeability") positiveCorrelation a(MESH:"Complement Membrane Attack Complex") View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

path(MESH:Hemolysis) positiveCorrelation bp(GO:"complement activation") View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

path(MESH:Hemolysis) positiveCorrelation a(MESH:"Complement Membrane Attack Complex") View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence a837a09f0c
JSON

Following complement cascade activation, MAC on the cell membrane forms a pore resulting in membrane permeability changes17 finally leading to RBC morphological alterations and erythrocyte lysis.

a(MESH:"Complement Membrane Attack Complex") positiveCorrelation path(MESH:Hemolysis) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Discussion

a(MESH:"Complement Membrane Attack Complex") positiveCorrelation bp(MESH:"Cell Membrane Permeability") View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Discussion

bp(MESH:"Cell Membrane Permeability") positiveCorrelation a(MESH:"Complement Membrane Attack Complex") View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Discussion

path(MESH:Hemolysis) positiveCorrelation a(MESH:"Complement Membrane Attack Complex") View Subject | View Object

Appears in Networks:
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MeSH
Cerebral Hemorrhage
Text Location
Discussion

Evidence 755e4ca19b
JSON

In this present study, MAC contents in the clot gradually increased with a marked accumulation at day-3 (53.5 ± 8.2 vs. 11.0 ± 3.9 ng/g at 4 hour, p<0.01, Fig. 2C) and stayed at high levels at day 7.

a(MESH:"Complement Membrane Attack Complex") positiveCorrelation path(MESH:Hematoma) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

path(MESH:Hematoma) positiveCorrelation a(MESH:"Complement Membrane Attack Complex") View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence 02049f41b4
JSON

We found that diameter of RBC decreased gradually in the hematoma edge and hematoma center (e.g. day-3 at hematoma edge: 2.71 ± 0.38μm vs. 3.89 ± 0.35μm at 4 hours, p<0.01; hematoma center: 2.61 ± 0.29 μm vs. 3.75 ± 0.25μm at 4 hours, p<0.01. Fig. 1).

bp(MESH:"Erythrocyte Indices") negativeCorrelation path(MESH:Hematoma) View Subject | View Object

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path(MESH:Hematoma) negativeCorrelation bp(MESH:"Erythrocyte Indices") View Subject | View Object

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Evidence 0e4109d5cd
JSON

The hematoma clearance may be via red blood cell (RBC) lysis or phagocytosis.

bp(MESH:Phagocytosis) negativeCorrelation path(MESH:Hematoma) View Subject | View Object

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Introduction

path(MESH:Hematoma) negativeCorrelation bp(MESH:Phagocytosis) View Subject | View Object

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Introduction

Evidence a6d3ebc4f3
JSON

The level of hemoglobin declined gradually over the time after the onset of ICH with a significant reduction at day 3. The contents of hemoglobin in the clot were 26.2 ± 5.2 mg/g at 4 hours, but were reduced to 13.9 ± 0.9 mg/g at day-3 (Fig. 2B, p<0.01).

p(HGNC:HBB) negativeCorrelation path(MESH:Hematoma) View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

path(MESH:Hematoma) negativeCorrelation p(HGNC:HBB) View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence 2435ed9450
JSON

CD163 and HO-1 positive cells infiltrated into the hematoma from edge to center after ICH (Figs. 4A & 4B). A significant increase of CD163 cells was found at day 3.

p(HGNC:CD163) positiveCorrelation path(MESH:Hematoma) View Subject | View Object

Appears in Networks:
Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

path(MESH:Hematoma) positiveCorrelation p(HGNC:CD163) View Subject | View Object

Appears in Networks:
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MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence c556ef8b8c
JSON

The current study examined the natural time course of CD47 expression in the hematoma in a swine ICH model and found that CD47 levels in the clot decrease with time.

p(HGNC:CD47) negativeCorrelation path(MESH:Hematoma) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Discussion

path(MESH:Hematoma) negativeCorrelation p(HGNC:CD47) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Discussion

Evidence 8e622036c3
JSON

Our recent study showed that depleting CD47 in RBCs resulted in faster hematoma clearance with microglia/ macrophages being less likely to phagocytize RBCs with CD47 than CD47-deficient RBC 7.

p(HGNC:CD47) negativeCorrelation path(MESH:Hematoma) View Subject | View Object

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Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
Text Location
Discussion

path(MESH:Hematoma) negativeCorrelation p(HGNC:CD47) View Subject | View Object

Appears in Networks:
Annotations
Cell Ontology (CL)
macrophage
MeSH
Cerebral Hemorrhage
Text Location
Discussion

Evidence 5f7ae3004d
JSON

HO-1 protein levels in the hematoma also increased at day-3 (HO-1/GAPDH: 0.65 ± 0.08 at day-3 vs. 0.04 ± 0.01 at 4 hour, p<0.01).

p(HGNC:HMOX1) positiveCorrelation path(MESH:Hematoma) View Subject | View Object

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MeSH
Cerebral Hemorrhage
Text Location
Results

path(MESH:Hematoma) positiveCorrelation p(HGNC:HMOX1) View Subject | View Object

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Annotations
MeSH
Cerebral Hemorrhage
Text Location
Results

Evidence e02d1e5392
JSON

Hematoma resolution occurs in the days after intracerebral hemorrhage (ICH).

path(MESH:"Cerebral Hemorrhage") positiveCorrelation path(MESH:Hematoma) View Subject | View Object

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Introduction

path(MESH:Hematoma) positiveCorrelation path(MESH:"Cerebral Hemorrhage") View Subject | View Object

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Introduction

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